­
­
­
­

Inventi Impact: Novel Excipients

Articles

  • Inventi:pne/14540/14
    Evaluation of In-vitro Toxicity of Peptide (N-acetyl-Leu-Gly-Leu-COOH)-substituted-?-cyclodextrin\nDerivative, a Novel Drug Carrier, in PC-12 Cells
    01-Jan-1970 Research 2014 : October - December
    Hamid Reza Sadeghnia, Faezeh Vahdati Hassani, Hamid Sadeghian, Maryam Miandehi, Farzin Hadizadeh, Gholamreza Karimi

    Background: Cyclodextrins (CDs) have been shown to improve physicochemical and biopharmaceutical\nproperties of drugs when low solubility and low safety limit their use in the pharmaceutical field. Recently, a new\namphiphilic peptide-substituted-?-CD, hepta-(N-acetyl-Leu-Gly-Leu)-?-CD (hepta-(N-acetyl-LGL)-?-CD), is\ndeveloped which exhibited good solubility, strong inclusion ability and an appropriate average molecular weight.\nHowever, there is limited information available about its toxic effects. This study was designed to evaluate\ncytotoxic effects of the hepta-(N-acetyl-LGL)-?-CD (50, 200, 400, and 800 ?g/ml) on rat pheochromocytoma PC-12\ncells.\nResults: A significant reduction of cell viability with IC50 values of 1115.0 ?g/ml, 762.4 ?g/ml, and 464.9 ?g/ml at 6,\n12, and 24 h post-treatment, respectively, as well as increased lipid peroxide levels and DNA damage were observed.\nConclusions: In conclusion, hepta-(N-acetyl-Leu-Gly-Leu)-?-CD exhibit significant toxic properties at high concentrations,\nprobably through induction of oxidative stress and genotoxicity.

    How to Cite this Article
    CC Compliant Citation: Sadeghnia et al.: Evaluation of in vitro toxicity of peptide (N-acetyl-Leu-Gly-Leu-COOH)-substituted-?-cyclodextrin derivative, a novel drug carrier, in PC-12 cells. DARU Journal of Pharmaceutical Sciences 2013 21:75,\ndoi:10.1186/2008-2231-21-75. This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
    Download Full Text